Shtapenko Oksana
Dr.Sc., Senior Scientist
Leading researcher, Laboratory of biotechnology of reproduction
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EDUCATION AND PROFESSIONAL EXPERIENCE
1991-1996 – Student of the Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytskyj.
1997-2000 – Post-graduate student of the Institute of Agriculture and Animal Biology of the Ukrainian Academy of Sciences (now Institute of Animal Biology NAAS, Lviv), specialty «Biochemistry».
2003-2004 – Research Assistant of the Cellular Engineering Laboratory, Institute of Animal Biology NAAS.
2004 – Ph. D. Thesis: «Regulation of oocytes maturation and cow’s reproductive functions with hormonal action in vitro and in vivo», Institute of Animal Biology NAAS, Lviv. Speciality: Biochemistry.
2005-2007 − Research Scientist of the Laboratory of Embryonic Biotechnology, later in 2008-2015 –transferred to the position of the Senior Scientist of this laboratory.
2013 − Senior Researcher Diploma in Biochemistry.
2015-2020 − Senior Scientist of the Laboratory of Reproductive Biotechnology, Institute of Animal Biology NAAS.
2020 – Dr. Sc. (habilitation) Thesis: «Biotechnological aspects of regulation of gametogenesis for correction of early embryonic development of animals», National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Ministry of Education and Science of Ukraine, Kyiv. Speciality: Biotechnology.
From 2021 – Leading researcher of the Laboratory of Reproductive Biotechnology, Institute of Animal Biology NAAS.
RESEARCH ACTIVITIES
Scholarship of the Cabinet of Minister of Ukraine for young scientists (2005-2006).
Fellowship National Cancer Institute, Bethesda (USA), Department of Tumor Biology, M.Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch (2009-2010).
Accreditation of the Doctoral Degree Program in the Biology 091 by the national Committee on Education of the National Agency for Higher Education Quality Assurance, Ukraine (guarantor of the educational and scientific program), 2021.
Member of Methodical Committee of the Institute of Animal Biology of NAAS.
Member of Ukrainian Biochemical and Physiological Societies.
SCIENTIFIC INTEREST
Study of spermato- and oogenesis, patterns of embryonic development and fertilizing ability of animals under the influence of exogenous factors of various nature.
Cell biotechnology: cultivation of cell lines and primary reproductive cell (oviducts, uterine cells culture) in vitro with control of cell proliferation, viability and metabolic activity; using the polymer nanolayers to enhance cell growth in vitro.
Reproductive biotechnology: in vitro manipulation techniques of oocytes, sperm and embryos.
The use of molecular biology methods in animal reproductive biotechnology.
Development of innovative preparations to improve reproductive capacity and fertility in farm animals.
Biotechnological potential and probiotic properties of newly isolated Enterococcus lactis strains.
MAIN PUBLICATION
Article
Shtapenko O. V., Gevkan I. I., Syrvatka V. Y., Slyvchuk O. Y., Korbetska O. O., Kornyat S. B., Yaremchuk I. M. Creation of combined stabilizing compositions to preserve the activity of gonadotropins in liquid form. The Animal Biology, 2022, 24(3): 14–17. https://doi.org/10.15407/animbiol24.03.014
Slyvchyk Yu. I., Shtapenko O.V. et al. Effects of copper nanoparticles on the biochemical parameters of blood serum of female rabbits. The Animal Biology, 2020, 22(1): 20–25. https://doi.org/10.15407/animbiol22.01.020
Syrvatka V., Slyvchyk Yu., Shtapenko O., Gromuko O., Gevkan I. Combined space-organized system for animals oocytes maturation in vitro. Factors of experimental evolution of organisms, 2020, 26:264-269. DOI: https://doi.org/10.7124/FEEO.v26.1277
Shtapenko O., Gevkan I., Sluvchyk Yu., Dzen` Ye., Syrvatka V., Matvienko N. Effect of organic microelements liposomal form on fertilizing ability and antioxidant status of female rabbits. Biotechnologia Acta, 2018; 11 (4); 50-56. https://doi.org/10.15407/biotech11.04.050
Shtapenko O. , Gevkan I., Sluvchyk Yu., Dzen` Ye., Syrvatka V., Matvienko N. Formation and properties polymer nanolayers to enhance cell growth in vitro. Innov. Biosyst. Bioen. 2018; 2(2): 105-109. DOI https://doi.org/10.20535/ibb.2018.2.2.132515
Shtapenko O., Matiukha I., Hevkan I., Tkachenko H. The influence of trace elements in the form of liposomal compounds on antioxidant defense system and parameters of oxidative stress in the reproductive organs of female rabbits. Slupskie Prace Biologiczne. 2015;12: 173-186. DOI https://spb.apsl.edu.pl/pliki/nr12/12.pdf
Shtapenko O., Gevkan I., Dzen` Ye., Fedorova S., Sluvchyk Yu., Syrvatka V., Matiukha I. Effect of liposomal preparate with some organic trace elements on antioxidant status and reproductive ability of female rabbits. Scientific Papers. Series D. Animal Science. 2015; Vol. LVIII; 221-227 DOI http://animalsciencejournal.usamv.ro/pdf/2015/pdf2015.pdf
Syrvatka V.J., Slyvchuk Y.I., Rozgoni I.I., Gevkan I.I., Shtapenko O.V. Sensitive and rapid assay for determination of protein concentration using silver nanoparticles with hyaluronan. Optics in the Life Sciences. OSA Technical Digest. 2015; OM4D.3. doi: https://doi.org/10.1364/OMP.2015.OM4D.3
Shtapenko O.V. Effect of hyperthermia on gene expression in mice tissues. Scientific journal “Visnyk of Lviv University” Biological Series. 2013; 62; 126-132.
Raczkowska J, Ohar M, Stetsyshyn Yu, Zemla J, Awsiuk K, Rysz J, Fornal K, Bernasik A, Ohar H, Fedorova S, Shtapenko O, Polovkovych S, Novikov V, Budkowski A. (2014) Temperature-responsive peptide-mimetic coating based on poly(N-methacryloyl-L-leucine): properties, protein adsorption and cell growth. Colloids and Surfaces B:Biointerfaces. 118, P. 270-279. DOI: 10.1016/j.colsurfb.2014.03.049
Monographs
“Living organisms and bioanalytical approaches for detoxification and monitoring of toxic compounds”. Сhapter in Living Organisms Approaches for Detoxification and Monitoring of Toxic Compounds edited by: A. Sibirny, D. Fedorovych, M. Gonchar, D. Grabek-Lejko. // Comparative toxicity screening of organic and inorganic copper salts on oviduct epithelial cell culture in vitro, O. Shtapenko, I. Hevkan, S. Fedorova, Yu. Slyvchuk, I. Matuha, V. Syrvatka. ISBN 978-83-7667-203-8 Zimowit Ltd. Rzeszow Poland 2015, P. 342.
Cell culture and its possible use in embryonic biotechnology. Madich A., Sheremeta V., Hevkan I., Shtapenko O., Fedorova S., Yu.Slyvchuk Manual for basic technique. — K.ArtEkom, 2012. — 144 p.
Methodical Instructions "Obtaining of Functional Nanosurfaces for Cell Cultivation, OocytesCumulus Complexes and Animal Embryos"/ O. V. Shtapenko, I. I. Gevkan, Yu. Stetsyshyn, S. Fedorova, Yu. I. Sluvchyk, V. Y. Syrvatka «ENCI VP CD». Lviv, 2015. 28 p.
Laboratory research methods in biology, animal husbandry and veterinary medicine: handbook / [Vlizlo V. V., Fedoruk R. S., Ratych I. B., Shtapenko O. V. and others]; for order. V. V. Vlizla. - Lviv: Spolom, 2012. - 764 p.
Patents
Technical Specifications of Ukraine TU 21.2-30995014-00:2018 Preparation "Lipoorganik" (Shtapenko O. V., Dzen` Ye. O.).
Patent of Ukraine No. UA148331, 2021. “Method of obtaining colloidal solution of copper nanoparticles”. Shtapenko OV, Gevkan II, Syrvatka VJ, Slyvchuk OY.
Patent of Ukraine No.138343 25.11.2019 "A preparation for stimulating of sexual activity and spermatogenesis in sheep" Gevkan I.I., Sharan M.M., Grymak H.M., Shtapenko O.V., et al.
Patent of Ukraine No. 119565 from 09.25.2017 "The method of stabilization of human chorionic hormone in the liquid form of the preparation" Slyvchuk Y.I., Gevkan I.I., Shtapenko O.V., SyrvatkaV.Ya.
Patent of Ukraine No. 105740 from 11.04.2016. "Liporganic" preparation for enhancing of reproductive ability of female rabbits" SHTAPENKO O.V., Gevkan I.I., Slyvchuk Y.I., Dzen’ Y.O., Syrvatka V.Ya., Matyukha I.O., Fedorova S.V.
Patent of Ukraine No u20108053from 25.01.2011. "Method of reducing the level of embryonic mortality in rabbits" Shtapenko O. V., Gevkan I. I., Dzen` Y.O., Fedorova S. V.
Patent of Ukraine u201005025 from 25.11.2010 "Environment to increase the activity of cell proliferation of different types" Slyvchuk Yu. I., Gevkan I. I., Shtapenko O. V., Fedorova S. V.
Patent of Ukraine u200904658 from 10.02.2010 "The method of obtaining feeder cells of the fallopian tubes" Madich A. V., Gevkan I. I., Shtapenko O. V., Slyvchuk Yu. I., Fedorova S. V.