Tetyana V. Buslyk
PhD in Biological Sciences, Senior Researcher (Specialization 204: Technology of Production and Processing of Livestock Products)
Leading Researcher, Laboratory of Molecular Biology and Genetics
This email address is being protected from spambots. You need JavaScript enabled to view it. , This email address is being protected from spambots. You need JavaScript enabled to view it.
Education and Professional Experience
2005: Graduated with honors from Ivan Franko National University of Lviv, majoring in Biochemistry.
2005–2008: Postgraduate studies (PhD program) at Ivan Franko National University of Lviv, specializing in Biochemistry (03.00.04).
2009: Defended PhD thesis: "Structural and functional organization of erythrocytes under conditions of type 1 diabetes mellitus" at the Specialized Scientific Council of the Institute of Animal Biology NAAS (Lviv). Awarded the degree of Candidate of Biological Sciences (PhD).
2010–2011: Research Scientist, Breeding Laboratory, O.V. Kvasnytskyi Institute of Pig Breeding NAAS.
2011–2012: Research Scientist, Genetics Laboratory, Institute of Pig Breeding and Agro-Industrial Production NAAS.
2012–2019: Senior Research Scientist, Genetics Laboratory, Institute of Pig Breeding and Agro-Industrial Production NAAS.
2019: Awarded the academic title of Senior Researcher in the specialty 204 "Technology of Production and Processing of Livestock Products."
2021–2025: Senior Research Scientist, S. Gzhytskyi Laboratory of Metabolism, Institute of Animal Biology NAAS.
2026–Present: Leading Scientific Researcher, Laboratory of Molecular Biology and Genetics, Institute of Animal Biology NAAS.
2005–2008: Postgraduate studies (PhD program) at Ivan Franko National University of Lviv, specializing in Biochemistry (03.00.04).
2009: Defended PhD thesis: "Structural and functional organization of erythrocytes under conditions of type 1 diabetes mellitus" at the Specialized Scientific Council of the Institute of Animal Biology NAAS (Lviv). Awarded the degree of Candidate of Biological Sciences (PhD).
2010–2011: Research Scientist, Breeding Laboratory, O.V. Kvasnytskyi Institute of Pig Breeding NAAS.
2011–2012: Research Scientist, Genetics Laboratory, Institute of Pig Breeding and Agro-Industrial Production NAAS.
2012–2019: Senior Research Scientist, Genetics Laboratory, Institute of Pig Breeding and Agro-Industrial Production NAAS.
2019: Awarded the academic title of Senior Researcher in the specialty 204 "Technology of Production and Processing of Livestock Products."
2021–2025: Senior Research Scientist, S. Gzhytskyi Laboratory of Metabolism, Institute of Animal Biology NAAS.
2026–Present: Leading Scientific Researcher, Laboratory of Molecular Biology and Genetics, Institute of Animal Biology NAAS.
Scientific Activity
Author and co-author of over 50 scientific publications, including 10 articles in peer-reviewed journals indexed in Scopus and Web of Science.
Research Interests
• Development of DNA technologies for livestock typing based on genes controlling economically valuable and biological traits.
• Identification of molecular markers for the implementation of Marker-Assisted Selection (MAS).
• Genetic monitoring of selection processes.
• PCR diagnostics of infectious diseases in animals.
Technical Skills
Expertise in molecular genetic analysis techniques, including:
• Restriction Fragment Length Polymorphism (PCR-RFLP), Real-Time PCR, and Microsatellite analysis.
• Various DNA extraction protocols and separation of amplified DNA fragments via electrophoresis (native and denaturing conditions).
• Immunoblotting and Lectin-blotting analysis.
• PCR primer design and optimization of amplification conditions.
• Cross-species experience: Research involving humans, domestic and wild pigs, moose, red deer, and various plant species and microorganisms.
• Identification of molecular markers for the implementation of Marker-Assisted Selection (MAS).
• Genetic monitoring of selection processes.
• PCR diagnostics of infectious diseases in animals.
Technical Skills
Expertise in molecular genetic analysis techniques, including:
• Restriction Fragment Length Polymorphism (PCR-RFLP), Real-Time PCR, and Microsatellite analysis.
• Various DNA extraction protocols and separation of amplified DNA fragments via electrophoresis (native and denaturing conditions).
• Immunoblotting and Lectin-blotting analysis.
• PCR primer design and optimization of amplification conditions.
• Cross-species experience: Research involving humans, domestic and wild pigs, moose, red deer, and various plant species and microorganisms.
Key Publications
Buslyk, T., Peka, M., Saienko, A., Kozak, M., Yuzviak, M., Salyha, Y., Stapay, P., Trapina, I., & Paramonova, N. (2026). Identification and bioinformatic functional analysis of novel and known polymorphisms in the myostatin gene of Ukrainian Carpathian Mountain sheep. Scientific reports, 10.1038/s41598-026-44326-6 https://doi.org/10.1038/s41598-026-44326-6 (Scimago Q1)
Buslyk, T., Kozak, M., Yuzviak, M., Salyha, Y., Stapay, P., Saienko, A., & Trapina, I., & Paramonova, N. (2025). Sequence analysis of the 3′UTR region of the myostatin gene in Ukrainian Carpathian Mountain sheep. The Animal Biology. 2025; 27(1), 3-8. https://doi.org/10.15407/animbiol27.01.003
Buslyk T.V., Rosalovsky V.P., Salyha Y.T. (2022). PCR-Based Detection and Quantification of Mycotoxin-Producing Fungi. Cytology & Genetics, 56 (1), 16–30. https://doi.org/10.3103/S0095452722010042 (Scimago Q4)
Balatsky, V. N., Oliinychenko, Y. K., Saienko, A. M, Buslyk, T. V., Bankovska, I. B., Peka, M. Yu., & Doran, O. (2022). Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed. Cytology & Genetics, 56, 513–525. https://doi.org/10.3103/S0095452722060020 (Scimago Q4)
Balatsky V.N., Oliinychenko Y.K., Buslyk T.V., Bankovska I.B., Korinnyi S.N., Saienko A.M., & Pochernyaev K.F. (2021). Associations of QTL Region Genes of Chromosome 2 with Meat Quality Traits and Productivity of the Ukrainian Large White Pig Breed. Cytology & Genetics, 55, 53–62. https://doi.org/10.3103/S0095452721010023 (Scimago Q3)
Stapay, P. V., Buslyk, T. V., Mykhaliuk, V. V., Stakhiv, N. P., Smolyaninova, O. O., Nevostruyeva, I. V., Sachko, R. G., Skorokhid, А. V., Pakholkiv, N. I. (2024). The ratio of structural proteins in the wool of sheep of different breeds and with different categories of fibers depending on the zones of their breeding. The Animal Biology, 26(1), 24–28. https://doi.org/10.15407/animbiol26.01.024
Stapai, P. Sachko, R., Pakholkiv, N., Buslyk, T., Skorokhid, A. (2024). Dependence of sulfur content and physical parameters of wool of ewe hoggets on their breed and breeding zones. Bulletin of Agricultural Science, 102(2), 43-47. https://doi.org/10.31073/agrovisnyk202402-07
Bankovska, I., Oliinychenko, Y., Balatsky, V., Buslyk, T., Hryshchenko, S., & Susol, R. (2020). Association of LEP- and CTSF-genotypes with levels of meat quality PSE, NOR and DFD in pigs of large white breed of Ukrainian selection. Agricultural Science and Practice, 7(1), 14-23. https://doi.org/10.15407/agrisp7.01.014 (Web of Science, Q4)
Viktor Balatsky, Irina Bankovska, Ramona N. Pena, Artem Saienko, Tetyana Buslyk, Sergii Korinnyi, Olena Doran. Polymorphisms of the porcine cathepsins, growth hormone-releasing hormone and leptin receptor genes and their association with meat quality traits in Ukrainian Large White breed // Mol Biol Rep. – 2016. – Vol. 43. – pp. 517–526.
Patents
Patent No. 141127 (2020): Method for identifying DNA fragments of Waddlia chondrophila bacteria via 16S rRNA gene amplification.
Patent No. 141128 (2020): Method for identifying DNA fragments of Parachlamydia acanthamoebae bacteria via 16S rRNA gene amplification.
https://www.scimagojr.com/journalsearch.php?q=19061&tip=sid&clean=0
Buslyk, T., Kozak, M., Yuzviak, M., Salyha, Y., Stapay, P., Saienko, A., & Trapina, I., & Paramonova, N. (2025). Sequence analysis of the 3′UTR region of the myostatin gene in Ukrainian Carpathian Mountain sheep. The Animal Biology. 2025; 27(1), 3-8. https://doi.org/10.15407/animbiol27.01.003
Buslyk T.V., Rosalovsky V.P., Salyha Y.T. (2022). PCR-Based Detection and Quantification of Mycotoxin-Producing Fungi. Cytology & Genetics, 56 (1), 16–30. https://doi.org/10.3103/S0095452722010042 (Scimago Q4)
Balatsky, V. N., Oliinychenko, Y. K., Saienko, A. M, Buslyk, T. V., Bankovska, I. B., Peka, M. Yu., & Doran, O. (2022). Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed. Cytology & Genetics, 56, 513–525. https://doi.org/10.3103/S0095452722060020 (Scimago Q4)
Balatsky V.N., Oliinychenko Y.K., Buslyk T.V., Bankovska I.B., Korinnyi S.N., Saienko A.M., & Pochernyaev K.F. (2021). Associations of QTL Region Genes of Chromosome 2 with Meat Quality Traits and Productivity of the Ukrainian Large White Pig Breed. Cytology & Genetics, 55, 53–62. https://doi.org/10.3103/S0095452721010023 (Scimago Q3)
Stapay, P. V., Buslyk, T. V., Mykhaliuk, V. V., Stakhiv, N. P., Smolyaninova, O. O., Nevostruyeva, I. V., Sachko, R. G., Skorokhid, А. V., Pakholkiv, N. I. (2024). The ratio of structural proteins in the wool of sheep of different breeds and with different categories of fibers depending on the zones of their breeding. The Animal Biology, 26(1), 24–28. https://doi.org/10.15407/animbiol26.01.024
Stapai, P. Sachko, R., Pakholkiv, N., Buslyk, T., Skorokhid, A. (2024). Dependence of sulfur content and physical parameters of wool of ewe hoggets on their breed and breeding zones. Bulletin of Agricultural Science, 102(2), 43-47. https://doi.org/10.31073/agrovisnyk202402-07
Bankovska, I., Oliinychenko, Y., Balatsky, V., Buslyk, T., Hryshchenko, S., & Susol, R. (2020). Association of LEP- and CTSF-genotypes with levels of meat quality PSE, NOR and DFD in pigs of large white breed of Ukrainian selection. Agricultural Science and Practice, 7(1), 14-23. https://doi.org/10.15407/agrisp7.01.014 (Web of Science, Q4)
Viktor Balatsky, Irina Bankovska, Ramona N. Pena, Artem Saienko, Tetyana Buslyk, Sergii Korinnyi, Olena Doran. Polymorphisms of the porcine cathepsins, growth hormone-releasing hormone and leptin receptor genes and their association with meat quality traits in Ukrainian Large White breed // Mol Biol Rep. – 2016. – Vol. 43. – pp. 517–526.
Patents
Patent No. 141127 (2020): Method for identifying DNA fragments of Waddlia chondrophila bacteria via 16S rRNA gene amplification.
Patent No. 141128 (2020): Method for identifying DNA fragments of Parachlamydia acanthamoebae bacteria via 16S rRNA gene amplification.
https://www.scimagojr.com/journalsearch.php?q=19061&tip=sid&clean=0
