Mariya Kozak
Ph.D. Senior researcher
Acting head of Laboratory of Molecular Biology and Genetics
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Education and professional activity
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post-transcriptional mRNA degradation for controlled inhibition of gene expression in vitro and in vivo;
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non-viral delivery systems for nucleic acids and proteins;
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the development and testing of new substances with adjuvant properties in order to create effective and safe vaccines;
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the development of new antimicrobial drugs and their testing.
Scientific activity
COST Action CA22112 European Network on Livestock Phenomics (EU-LI-PHE) Working Groups 2 and 3 https://e-services.cost.eu/action/CA22112
Scientific projects
0125U001633 “New synthetic substances with antimicrobial properties for treating wounds”, Institute of Animal Biology of NAAS of Ukraine, 2025, state budget funds
https://nrat.ukrintei.ua/searchdoc/0226U001846/
0126U002016 “Biological properties and mechanism of action of new polymeric adjuvants”, Institute of Animal Biology of NAAS of Ukraine, 2026-2030, state budget funds
https://nrat.ukrintei.ua/searchdoc/0126U002016
NATO SPS Project - G6107 Polymer hydrogels for safe and efficient vaccines, international, organizer -University of Health Sciences in Turkey, implementors of the project - Lviv Polytechnic National University, Institute of Cell Biology of the National Academy of Sciences of Ukraine and Institute of Animal Biology of NAAS of Ukraine, funding - North Atlantic Treaty Organization (NATO), 2024-2027, https://lpnu.ua/en/news/lviv-polytechnic-researchers-will-develop-innovative-vaccines-support-nato-grant-program
Project participant, EURIZON FELLOWSHIP PROGRAMME Remote Research Grants ID#3048 «Comparative study of Latvian and Ukrainian breeds of sheep by SNP markers involved in the formation of economically useful traits», international, organizer and implementation - Institute of Animal Biology of NAAS of Ukraine, funding – European Union (through EURIZON H2020), 2024-2025, https://indico.desy.de/event/38700/
• post-transcriptional mRNA degradation for controlled inhibition of gene expression in vitro and in vivo;
• non-viral delivery systems for nucleic acids and proteins;
• the development and testing of new substances with adjuvant properties in order to create effective and safe vaccines;
• the development of new antimicrobial drugs and their testing;
• development and efficacy evaluation of new antimicrobial agents and delivery systems for therapeutic compounds;
• identification of genes responsible for the formation of productive qualities in farm animals;
• analysis of candidate gene polymorphisms for marker-associated breeding to improve economically useful traits in animals.
1. Buslyk, T., Peka, M., Saienko, A., Kozak, M., Yuzviak, M., Salyha, Y., Stapay, P., Trapina, I., & Paramonova, N. (2026). Identification and bioinformatic functional analysis of novel and known polymorphisms in the myostatin gene of Ukrainian Carpathian Mountain sheep. Scientific reports, https://doi.org/10.1038/s41598-026-44326-6 (Scopus, Q1)
2. Kozak, M., Petruh, I., Kovalchuk, I., & Vlizlo, V. (2025). Toxicity analysis of amoxicillin, polyphosphate ester and its complex with amoxicillin on mice. Scientific reports, 15(1), 18150. https://doi.org/10.1038/s41598-025-94916-z (Scopus, Q1)
3. Buslyk, T., Kozak, M., Yuzviak, M., Salyha, Y., Stapay, P., Saienko, A., & Trapina, I., & Paramonova, N. (2025). Sequence analysis of the 3′UTR region of the myostatin gene in Ukrainian Carpathian Mountain sheep. The Animal Biology. 2025; 27(1), 3-8. https://doi.org/10.15407/animbiol27.01.003
4. Kozak, M., Sementsiv, K., Sementsiv N., Petruh, I. (2025). Formulation and Wound Healing Evaluation in vivo of Ointment Based on Propolis, and Containing Curcumin. Majalah Obat Tradisional, 30(3), 347–354. http://doi.org/10.22146/mot.104987. (Scopus, Q4)
5. Kozak M., Stasiuk A., Vlizlo V., et al. (2023) Polyphosphate Ester-Type Transporters Improve Antimicrobial Properties of Oxytetracycline Antibiotics, 12(3), 616. https://doi.org/10.3390/antibiotics12030616 (Scopus, Q1)
6. Kozak, M., Zelenina, O., Ostapiv, D., et al. (2023). Blood creatinine content and rat kidney structure after intramuscular injection of pegylated antibiotic enrofloxacin. Studia Biologica, 17(3), 47–56. http://dx.doi.org/10.30970/sbi.1703.720(Scopus, Q4)
7. Zelenina O., Vlizlo V., Kozak M., et al. (2022) Antimicrobial activity of the PEGylated antibiotic enrofloxacin and its functional and structural effect on the liver in rats. Journal of Applied Pharmaceutical Science, 12(06), https://dx.doi.org/10.7324/JAPS.2022.120607 (Scopus, Q2).
8. Kozak M., Petruh I., Vlizlo V. (2022) Comparison of adjuvant properties of chitosan during oral and subcutaneous immunization of mice with BSA. Ukrainian Biochemical Journal, 94(2): 31–37. https://doi.org/10.15407/ubj94.02.031 (Scopus, Q4).
9. Vudmaska, I., Petrukh, I., Sachko, S., Vlizlo, V., Kosenko, Y., Kozak, M., Petruk, A. (2021). Using hop cones, vitamin E, methionine, choline and carnitine for treatment of subclinical ketosis in transition dairy cows. Advances in Animal and Veterinary Sciences, 9(1), 55-62. http://dx.doi.org/10.17582/journal.aavs/2021/9.1.55.62 (Scopus, Q3)
10. Ostapiv, D. D., Kuz’mina, N. V., Kozak, M. R., Hu, S. et al. (2021). Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases. Ukrainian Biochemical Journal, 93(1), 104-112. https://doi.org/10.15407/ubj93.01.104 (Scopus, Q4)
11. Vlizlo V., Mysak A., Stybel V., Voloshyn R., Leno Yu., Kuz'mina N., Kozak M., Bodnar Yu., Petpuh I., Ostapiv D., Dron I., Samaryk V., Bukartyk N., Nosova N., Varvarenko S. Treatment of postoperative wounds using pectin-based reinforced dressings and their antimicrobial activity. (2021). Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences. Vol 23 No 104 (2021): 41-46. https://nvlvet.com.ua/index.php/journal/article/view/4284
12. Kozak M. R., Ostapiv D. D., Mitina N. Y., Petruh I. M., Volianiuk K. A., Zaichenko A. S., Vlizlo V. V. (2021) An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration. Biopolym. Cell. 2021; 37(5):357-368. https://www.biopolymers.org.ua/content/37/5/357/ (Scopus, Q3)
13. Zelenina O.M., Vlizlo V.V., Ostapiv D.D., Samaryk V.Ja., Dron I.A., Kozak M.R., Kuzmina N.V., Chernushkin B.O., Maksymovych I.A., Leno M.I., Rusyn V.I., Prystupa O.I., Fedorovych V.L., Lukashchuk B.O., Zinko H.O. (2021) PEGylation of antibiotic enrofloxacin and its effects on the state of the antioxidant system in rats. Ukrainian Journal of Ecology, 2021, 11(1),202-208. xx-xx, doi: 10.15421/2020_30x.
14. Kozak, M., Mitina, N., Zaichenko, A., & Vlizlo, V. (2020). Anionic Polyelectrolyte Hydrogel as an Adjuvant for Vaccine Development. Scientia Pharmaceutica, 88(4), 56. https://doi.org/10.3390/scipharm88040056 (Scopus, Q2) https://www.ujecology.com/articles/pegylation-of-antibiotic-enrofloxacin-and-its-effects-on-the-state-of-the-antioxidant-system-in-rats.pdf
15. Stetsko T. I., Muzyka V.P., Kozak M.R. (2020) Mechanisms of Antimicrobial Resistance in Bacteria. Theoretical and Applied Veterinary Medicine. Vol. 8(3), 2020 file:///C:/Users/MolBio/Downloads/287-Article%20Text-558-1-10-20201227%20(1).pdf
16. Kushkevych M.V., Kozak M.R., Petruh I.M., Vlizlo V.V. (2018) Сellular prion level in the animals’ tissues. Veterinary Biotechnology. 2018, 32 (2), 611-616. https://doi.org/10.31073/vet_biotech32(2)-75
17. Kozak M., Oliynyk A., Moskvin М., Ostapiv D., Mitina N., Zaichenko O., Vlizlo V. (2017). Anionic Polyelectrolyte Hydrogels: Influence on Antibodies Production and Enzyme Activity ECPB 2017, 79(3): 11–16. https://doi.org/10.25040/ecpb2017.03
18. Kozak M. R., Oliynyk A. V., Zaichenko O. S., Vlizlo V. V. (2013) Adjuvant properties of polymer based on acrylic acid. 2013, 85 (3): 69 – 73 http://ukrbiochemjournal.org/wp-content/uploads/2015/10/Kozak_3_13.pdf (Scopus Q4)
19. Vlizlo V. V., Zaichenko O. S., Ivanytska L. A., Kozak M. R., Ostapiv D. D. (2013). Detection of complexes of oligodeoxynucleotides with polymeric carriers. Biotechnologia acta, 2013, 6(5):94-99 https://biotechnology.kiev.ua/images/BTA/2013/5_2013/vlizlo_5_2013.pdf
20. Kozak M. R., Vlizlo V. V., Kit Y. Y., Stoika R. S.(2008) Induction of apoptosis and necrosis in leukemic cells by purified IgG of blood serum of mice which were fed with cattle brain for a long time. Biopolym. Cell. 2008; 24(1):28-34. https://doi.org/10.7124/bc.00078D (Scopus, Q3)
