The Laboratory of Metabolism was founded in 1960 by Dr.Sc., Prof. Stepan Gzytsky, who managed the laboratory until 1976. The next head of the laboratory was: from 1976 to 1997 Dr.Sc., Prof. Hryhoriy Kalachniuk; from 1997 to 2007: Dr.Sc., Prof. Leonid Solohub;  from 2007 to 2019 Dr.Sc. Senior Researcher Yuriy Salyha. Since January 2020 the laboratory head is Dr.Sc., prof. Petro Stapay.


Stapay Petro – Head of the laboratory, Dr.Sc., professor

Dzen Yevhen – leading researcher, PhD

Buslyk Tetiana – senior researcher, PhD

Sachko Roman – senior researcher , PhD

Nevostruyeva Iryna – senior researcher , PhD

Rosalovsky Volodymyr – senior researcher, PhD

Skorokhid Andriy – researcher, PhD

Stakhiv Nadia – researcher, PhD

Mykhaliuk Vasylyna – researcher, PhD

Savyak Zoryana – senior specialist

PhD students:

Perederiy Diana

Nazar Khrystyna


The laboratory performs the following tasks in the scientific research program 43 "Adaptation processes in highly productive farm animals under the influence of environmental and climatic factors":

    • F. To study the influence of environmental and climatic factors on the metabolism of animals and to develope methods of preventing metabolic disorders (2021-2025);
    • P. To investigate the ecobiological features of the wool sheeps of different breeds depending on the climatic zones of their breeding (2021-2023).

Study of physiological and biochemical changes of various body systems under the influence of environmental and climatic factors.

Search for genes responsible for the formation of productive qualities in farm animals.

Analysis of candidate gene polymorphisms for the possibility of their use in marker-associated selection to improve economically beneficial traits in animals.

Study of the physiological and biochemical foundations of the nutrition of ruminants with the aim of developing scientifically based measures to increase the bioconversion of the feed into the animal high-quality products - milk, meat, wool.

Study of the impact of climate change and heat stress on the metabolism and productivity of farm animals and poultry.

The influence of heat stress on the liver function of animals and the search for effective hepatoprotective agents under extreme temperature conditions and subcellular ketosis.

Study of physical characteristics (fineness, strength, solubility) of sheep wool of different breeds depending on their breeding zones.

Study of the protective properties of wool wax, its lipid composition under the influence of environmental factors.

Study of the structure and chemical composition of sheep wool of different breeds depending on their breeding zones.

Elucidation of the role of structural wool lipids of in the formation of physicochemical and technological properties of wool fibers.

Development of methods for evaluating wool formation processes and recommendations for the production of environmentally friendly wool raw materials for the health of consumers.


Foundation of the Ukrainian Research Institute of Farm Animals Physiology and Biochemistry in Lviv became possible due to the high authority of the worldwide known Ukrainian biochemist Stepan Gzhytsky, and the Lviv School of Biochemists that he formed.

TS. Gzhytsky Laboratory of Metabolism is accredited in terms of the level of laboratory equipment, methodical support and personnel training, meets industry requirements for conducting analytical and test studies of raw materials, products, biological material for compliance with regulatory documentation and providing practical consultations No. 029/ lab prod. dated 16.05.2018 by the State Research Control Institute of Veterinary Medicines and Feed Additives and the State Service of Ukraine for Food Safety and Consumer Protection of the Ministry of Agrarian Policy and Food of Ukraine.

The laboratory has developed a number of recommendations on various issues of the animal breeding industry, aimed at increasing the productivity and improving the quality parameters of their products.

The most significant contribution of those researches was determining the role of rumen in general metabolism and studies of the symbiosis between ruminants and microorganisms inhabiting the rumen. The priority research areas were: symbiotic processes in ruminants, physiological and biochemical bases for protein and energetic nutrition of animals, improving the quality and preservation of nutritional value of feed. Upon the initiative of academician H. Bohdanov, L. Solohub supervised the first research into methanogenesis in rumen of ruminantia and the emission of methane by ruminantia into the atmosphere. Those studies were very interesting both from ecological and economic standpoint and were aimed at discovering the impact of regional and agrotechnical factors on methanogenesis of ruminantia and the development of ways for its regulation.

The main directions of scientific activity are: study of physiological, biochemical, toxicological and neurobiological features of the effect of certain organophosphorus compounds (in particular chlorpyrifos) on the development and functioning of the central nervous system, the study of certain mechanisms of neurogenesis in norm and in pathology, and the study of ecological and bio- medical safety problems in the use of pesticides. Received by Y. Salyha results allow to form a fundamental understanding of the physiological and biochemical characteristics of effects on rats at the different levels of organization — from single cell to whole organism. An enhanced and complemented by pathway of physiological and biochemical mechanisms of chlorpyrifos toxicity, which is not limited to generally known anticholinergic effects, but is also closely linked to the phenomenon of oxidative stress and metabolism of biologically active metals in body tissues was proposed.

These are, in particular, recommendations for the prevention and elimination of such wool defects as yellowing, shedding and pathological thinning ("hungry fineness") of wool fibers, the use of macro- and microelements, amino acids, rapeseed fodder, filter perlite and dry apple pomace in sheep feeding.

Comprehensive biochemical studies have revealed a number of marker tests that positively correlate with performance indicators and, above all, with wool shearing. Such markers include lactate dehydrogenase (special subunit A), phospholipids, and the intensity of endogenous respiration. Also, the study has examined the processes of melanogenesis in the skin of sheep and developed recommendations for breeding Mountain Carpathian sheep with colored wool. Laboratory employees are co-authors of the creation of the Ukrainian Mountain Carpathian breed of sheep and a new breed type of Transcarpathian finewool sheep.


Study of physiological and biochemical changes of various body systems under the influence of environmental and climatic factors.

Search for genes responsible for the formation of productive qualities in farm animals.

Study of the physiological and biochemical basis of the nutrition of ruminants with the aim of developing scientifically based measures to increase the bioconversion of the feed into the animal high-quality products - milk, meat, wool.

The laboratory offers:
● physiological and biochemical studies of blood and muscles of farm animals and poultry;
● advisory assistance to farms on feeding animals of different species, age and productive groups;
● analysis of the forrage base for farm animals;
● conducting research in feed, feed mixtures and grain components.


Chhugani K, Frolova A, Salyha Y, Fiscutean A, Zlenko O, Reinsone S, et al. Remote opportunities for scholars in Ukraine. Science 2022;378(6626):1285-1286. DOI: 10.1126/science.adg0797 (Scopus)

Mykhaliuk VV, Havryliak VV, Salyha YT. The Role of Cytokeratins in Ensuring the Basic Cellular Functions and in Diagnosis of Disorders. Cytol. Genet. 2022;56(6):530-540.DOI: https://doi.org/10.3103/S0095452722060093

Salyha N, Salyha Y. L-glutamic acid mitigates carbon tetrachloride-induced acute tissue injury by reducing oxidative stress in a rat model. Current Chemical Biology. 2022; 16 (2): 130–137. DOI: 10.2174/2212796816666220408104856

Buslyk TV, Rosalovsky VP, Salyha YT. PCR-Based Detection and Quantification of Mycotoxin-Producing Fungi. Cytol Genet 2022;56(1):16-30. DOI: https://doi.org/10.3103/S0095452722010042

Havryliak V, Mykhaliuk V, Kochubei V. Characteristics of regenerated keratin and keratin-based film. Biopolym. Cell. 2021; 37(5): 335-345. DOI: http://dx.doi.org/10.7124/bc.000A5F

Rumynska T, Hural A, Konechnyi Y, Vynnytska R, Lozynskyi A, Salyha Y, Korniychuk O, Lesyk R. Microbial biofilms and some aspects of anti-inflammatory drug use. Biopolym. Cell. 2021; 37(4): 247-258. DOI: http://dx.doi.org/10.7124/bc.000A57

Vudmaska I, Petrukh I, Sachko S, Vlizlo V, Kosenko Y, Kozak M, Petruk A. Using hop cones, vitamin E, methionine, choline and carnitine for treatment of subclinical ketosis in transition dairy cows. Adv. Anim. Vet. Sci. 2021; 9(1): 55-62. DOI: http://dx.doi.org/10.17582/journal.aavs/2021/

Havryliak V, Mykhaliuk V, Petrina R, Fedorova O, Lubenets V, Novikov V. Adsorbents based on keratin for lead and cadmium removal. Current Applied Science and Technology. 2020; 20(1): 136-143

Salyha N, Salyha Y. Protective role of l-glutamic acid and l-cysteine in mitigation the chlorpyrifos-induced oxidative stress in rats. Environ Toxicol Pharmacol. 2018; 64: 155-163. DOI: http://dx.doi.org/10.1016/j.etap.2018.10.010

Hrabovska SV, Salyha YT. Animal Models of Autism Spectrum Disorders and Behavioral Techniques of their Examination. Neurophysiology 2016;48(5):380-388. DOI: DOI: http://dx.doi.org/10.1007/s11062-017-9613-2


S. Gzhytsky Laboratory of Metabolism

Contact person: Petro Stapay – Head of the laboratory, Dr.Sc. professor

Tel: +38 (063) 44 30 921

Email:  This email address is being protected from spambots. You need JavaScript enabled to view it.




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